Characterization of different high molecular weight angiotensinogen forms

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Characterization of different high molecular weight angiotensinogen forms.

BACKGROUND Angiotensinogen is the substrate for renin in the system that releases angiotensin II. This renin-angiotensin system is an important regulator of blood pressure (BP), and defects in the system are linked to the development of hypertension. Native angiotensinogen is a 62,000-dalton monomer, but various high molecular weight forms also exist, which have not been well characterized. Hig...

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Quantitation of five forms of high molecular weight angiotensinogen from human placenta.

In addition to the well characterized predominant form of plasma angiotensinogen, which will be termed low molecular weight angiotensinogen (LMrA), significant quantities of a high molecular weight angiotensinogen (HMrA) occur in the human pregnant state. HMrA is the predominant form of angiotensinogen in the placenta and amniotic fluid. The ratio of HMrA/LMrA is elevated in approximately half ...

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High molecular weight angiotensinogen levels in hypertensive pregnant women.

An apparent high molecular weight angiotensinogen (H-Aogen) can be separated from the usually predominant low molecular weight angiotensinogen (L-Aogen) by gel filtration of plasma. H-Aogen has been quantitated in plasma from normotensive menstruating women, estrogen treated women, normotensive pregnant women, women with pregnancy-induced hypertension (PIH), and women whose preexisting hyperten...

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Algal ferredoxin-NADP+ reductase with different molecular-weight forms.

Ferredoxin-NADP+ reductase from the microalga Bumilleriopsis was isolated by a combination of affinity chromatography on a flavodoxin-Sepharose 4 B column and usual purification procedures. Both the elution pattern of the final gel filtration step and of the sodium dodecylsulfate (SDS) disc gel electrophoresis indicate that there are at least two different molecular-weight forms of the reductas...

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Differential modulation of cell phenotype by different molecular weight forms of basic fibroblast growth factor: possible intracellular signaling by the high molecular weight forms

To study possible functional differences of the 18-kD and high molecular weight forms of basic fibroblast growth factor (bFGF), we have examined the effect of endogenous production of different bFGF forms on the phenotype of NIH 3T3 cells. Cells transfected with cDNAs coding for either 18-kD bFGF (18-kD bFGF) or all four molecular forms (18, 22, 22.5, 24 kD; wild type [WT] bFGF) exhibit increas...

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ژورنال

عنوان ژورنال: American Journal of Hypertension

سال: 2003

ISSN: 0895-7061

DOI: 10.1016/s0895-7061(03)00053-0